This AbVideo demonstrates step by step of the agarose gel preparation for DNA electrophoresis. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length.
RNA electrophoresis is an analytical technique used to separate RNA fragments. RNA has the tendency to form both secondary and tertiary structures that can impede its separation. So a denaturing condition is performed in formaldehyde and MOPS buffer system. RNA under this condition is fully denatured and migrates according to its molecular weight.
The quality of RNA preparation could be measured by electrophoresis on a denaturing agarose gel. Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the formaldehyde agarose gel for the electrophoresis of RNA.
SDS-PAGE is a technique used to separate proteins according to their electrophoretic mobility. SDS gel electrophoresis of samples have identical charge per unit mass due to binding of SDS results in fractionation by size, visualize the separated proteins, or process further application.