by Abnova
Aliquoting and Preparation of Serum before Usage
This is recommended to creat aliquots of serum when only small volumes are used at a time. This video shows you how to thaw the serum, aliquot into the 50ml tubes and refreeze.
Publish: 2011/5/31
Cell Lysate Preparation (Native)
Here we display you the protocol on how to prepare cell lysate. This includes 3 steps: First, cells are harvested. Then, cells are lysed in lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.
Publish: 2015/9/24
Tissue Lysate Preparation (Denatured)
This video demonstrates tissue lysate preparation when denatured and involves three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, denatured lysate in 1x Sample Buffer.
Publish: 2010/6/7
Tissue Lysate Preparation (Native)
Tissue lysate preparation is explained in this protocol which exemplifies the procedure with three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.
Publish: 2010/6/7