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AbVideo™ - Cell Lysate Preparation (Denatured)
- Publish: 2015/12/31
- Time: 2:24
by Abnova
Denaturation is a process in which proteins or nucleic acids lose their native structure due to external stress or compound. This AbVideo shows the protocol involves three steps for denatured lysate preparation. First, harvest the cells. Then, lyse the cells in the lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, denatured lysate in 1x Sample Buffer.
Related Video
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Aliquoting and Preparation of Serum before Usage
This is recommended to creat aliquots of serum when only small volumes are used at a time. This video shows you how to thaw the serum, aliquot into the 50ml tubes and refreeze.
Publish: 2011/5/31
-
Cell Lysate Preparation (Native)
Here we display you the protocol on how to prepare cell lysate. This includes 3 steps: First, cells are harvested. Then, cells are lysed in lysis buffer. After ultra speed centrifuge, cell lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.
Publish: 2015/9/24
-
Tissue Lysate Preparation (Denatured)
This video demonstrates tissue lysate preparation when denatured and involves three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, denatured lysate in 1x Sample Buffer.
Publish: 2010/6/7
-
Tissue Lysate Preparation (Native)
Tissue lysate preparation is explained in this protocol which exemplifies the procedure with three steps: First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as ready-to-use, native lysate in modified RIPA Buffer.
Publish: 2010/6/7
