This video demonstrates how to produce adenoviral vector after adenoviruses infection. Adenoviruses are double-stranded DNA viruses that cause respiratory, intestinal, and eye infections. The adenovirus vector system has been used for gene therapy.
cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.
Lentivirus is a genus of slow viruses of the Retroviridae family that have long incubation periods and cause chronic, progressive, often fatal diseases affecting many organs in humans. This video shows you how to infect culture cells with lentivirus. It is an important step before the production of lentiviral vector.
Lentiviruses are able to deliver genes into almost any mammalian cell type and have the ability to replicate in non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. This video presents the process of lentiviral production in 6cm plate.
This video presents the protocol for relative titer method for lentivirus. Lentivirus titers are estimated as relative infectious units (RIU), according to the curve of cell viability versus viral dose.
Hepatitis B viruses (HBV) are double-stranded DNA viruses that cause acute and chronic hepatitis. Furthermore, HBV infection is the leading cause of liver cancer. This video shows the procedure for using particle blot to analyze HBV Particle.
The plaque assay is a widely used approach for purifying a clonal population of virus and determining the viral titers (the lowest concentration of virus that results in infection). This video shows the procedure of plaque assay for influenza virus.