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AbVideo™ - 2D Gel Electrophoresis (3) Sample Preparation for the First Dimension

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  • Publish: 2011/3/25
  • Time: 5:03

by Abnova  

Learn how IEF Optimizer can be used for measuring sale content of the protein samples and calculating the suggested total voltage hour for IEF process with online program. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight).


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