AbVideo™

Gene Map Viewer

Map Viewer allows you to view and search complete genome of an organism, display chromosome maps, and zoom into progressively greater levels of detail, down to the sequence data for a region of interest.

Publish: 2010/8/19

Isolation of cccDNA

cccDNA (covalently closed circular DNA) is a crucial intermediate that arises in the cell nucleus during the propagation of hepadnaviruses. It may permit the persistence of virus infection. This video shows you how to isolation cccDNA form infected cells.

Publish: 2011/6/29

Cell Proliferation Assay (MTS)

CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound (MTS). The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at 490nm.

Publish: 2011/3/21

DNA Extraction by Phenol/Chloroform

This method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing phenol and chloroform, resulting in an upper aqueous phase and a lower organic phase (mainly chloroform). DNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

Publish: 2011/1/6

Lactate Dehydrogenase Assay Kit

Lactate Dehydrogenase Assay Kit is a colorimetric kinetic determination of lactate dehydrogenase activity. Lactate Dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. This video shows the procedures of the kit.

Publish: 2012/4/5

FastLink HRP Labeling Kit

The FastLink HRP Labeling Kit allows conjugations to set up in seconds, simply by adding a solution of the protein to be labeled to a proprietary lyophilised HRP mixture. By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, FastLink technology can be used to label small quantities of protein with 100% recovery and no excessive dilution of the conjugate. This video shows you how to set up conjugation reactions.

Publish: 2011/6/29

Website Tutorial - Product Comparison

This tutorial teaches you how to compare products on Abnova website. You can choose at least 2 products (up to 10) to compare. It shows specifications of each product. Then choose the best product for your research.

Publish: 2010/9/23

IHC Kit - RealBlue Peroxidase Substrate Kit

RealBlue Peroxidase Substrate kit is 50-100 times more sensitive than DAB and is non-carcinogenic. Strongly recommended for the detection of least-abundant markers. RealBlue forms a blue chromogenic product, providing excellent contrast with DAB and other substrates for multiple labeling experiments

Publish: 2010/12/10

mRNA Extraction

Due to the low proportion of mRNA (only 1~5% of the total RNA), reducing the amount of rRNA and tRNA in a total RNA preparation increases the relative amount of mRNA. mRNA enrichment is essential for construction of cDNA libraries and other applications where intact mRNA is highly desirable. This video shows how to use affinity resin to isolate mRNA.

Publish: 2010/4/19

Gel Extraction

Gel extraction is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. Three basic steps are involved: slicing the bands of interest on UV light exposure, isolating the DNA from those bands, and removing the accompanying salts and stain.

Publish: 2010/5/24

Derivation of Human Umbilical Vein Endothelial Cells (HUVEC)

Human umbilical vein endothelial cells (HUVEC) are commonly used as a laboratory model system for the physiological and pharmacological investigations. This video describes how to derive HUVEC from the endothelium of veins of the umbilical cord.

Publish: 2013/3/29

CytoQuest™ CR CTC Enrichment - Capture Ab Incubation

Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs), including circulating tumor cells (CTCs), circulating stem cells (CSCs), and circulating fetal cells (CFCs). Please visit Abnova website for more information.

Publish: 2014/1/28

2D Gel Electrophoresis (12) Mass Analysis

Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight). This video shows the part 12- mass analysis.

Publish: 2011/5/17

Micropipette Calibration

Micropipettes are used to accurately measure and dispense small volumes of liquid. The capacity of a micropipette can range from less than 1μl to 1000μl (1ml). To ensure that obtained results using micropipette are reliable, it is necessary to calibrate micropipettes several times a year. This video shows a method for testing the accuracy of automatic micropipettes.

Publish: 2010/7/19

2D Gel Electrophoresis (2) Protein Quantitation

Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight). This video shows the part 2- protein quantitation.

Publish: 2011/3/25

Antibody Conjugation - Biotinylation

Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction. A biotin/streptavidin (avidin) system offers low background, enhanced sensitivity and the ability to detect minute amounts of antigens.

Publish: 2010/4/26

Immunofluorescence

Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope.

Publish: 2009/12/13

Flow Cytometry - Double Stain

Flow cytometry is a technique for measurement and examination of microscopic particles in a flow system, which delivers particles singly past by an electronic detection apparatus. This video shows the procedures for flow cytometry analysis of cells using double staining.

Publish: 2011/8/2

Mycoplasma Detection: Direct Method

One of the most important cell culture procedures is using microtest to determine if mycoplasmal contaminant is present. This video demonstrates the steps of direct method for detection of Mycoplasma in the cell samples.

Publish: 2011/8/8

Prepare hESCs for Cryopreservation

Human embryonic stem cells (hESCs) are pluripotent cells are able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. This video describes how to prepare cryopreserved hESCs for long-term storage.

Publish: 2012/6/7