by
Abnova
Abnova offers a variety of arrays for protein expression profiling. These arrays are designed for researchers to study highly relevant proteins in the specific research fields like Angiogenesis, Apoptosis, Cancer Marker, Cell Cycle, Cytokine, Hematopoiesis, Hormone, Signal Transduction and Stem Cell.
by
Abnova
A powerful platform for protein-protein interaction and protein phosphorylation studies in situ is created by combining Abnovas dual recognition, primary antibody pair with proximity ligation assay from Olink Bioscience. This revolutionary combination enables unprecedented specificity and sensitivity of protein detection and quantification for high performance immunofluorescence and immunohistochemistry applications.
by
Abnova
Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.
by
Abnova
Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.
by
Abnova
Hemocytometer or Cell Counting Chamber is frequently used to count cell numbers such as cell density of cultured cells in research labs and blood count and sperm count in clinical labs. For counting of attached cells, cells are trypsinized, mixed with a dye (Trypan Blue), loaded onto a hemocytometer and then counted under a microscope.
by
Abnova
Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture attached cells from cell thawing, cell maintenance, to cell freezing.
by
Abnova
Cell culture is the process by which cells are grown under controlled conditions. This video shows you how to culture suspension cells from cell thawing, cell maintenance, to cell freezing.
by
Abnova
DNA sequencing is a technique to determine the order of the nucleotide bases-adenine, guanine, cytosine, and thymine. It is indispensable for basic biological research and discovery.
by
Abnova
Dot blot is a technique can be used as a semiqualitative method for rapid screening of a large number of samples. It is for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane.
by
Abnova
Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. We use GST-fusion protein to purify and detect proteins of interest. The GST-fusion protein can be purified from cells via its high affinity for glutathione.
by
Abnova
Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope.
by
Abnova
Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
by
Abnova
Magnetic beads conjugated with different ligands like antibodies, proteins or tags are used in Precipitor™ system and other applications such as immunoprecipitation (IP, ChIP, RIP), protein purification and protein-protein interaction to accelerate your research!
by
Abnova
Photon™ uses a state-of-the-art, photon-counting multiplier tube as the detector element to detect very small amounts of analytes, in the range of attomoles, by counting the number of photons generated from the chemiluminescent or bioluminescent experiment.
by
Abnova
Polymerase Chain Reaction (PCR) is a technique to amplify few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. There are three major steps (Denaturation, Annealing and Extension) in a PCR and repeat for 30 or 40 cycles.
by
Abnova
Precipitor™ system, an automated magnetic bead platform for high throughput precipitation and purification of proteins. It easily handles 16 different assays simultaneously and addresses the needs of rigorous proteomic screening and biomarker discovery applications such as immunoprecipitaton (IP, ChIP, RIP), recombinant protein purification, and protein-protein interaction.
by
Abnova
Protein can be directly quantitated by colorimetric. Coomassie Blue dye binds to peptides in an acidic medium. The color change of this assay is measured at 595 or 600 nm. We calculate the protein concentration using the formula created by standards.
by
Abnova
Protein can be directly quantitated using UV absorbance (280nm). 280nm Absorbance depends on the Tyr and Trp (amino acids with aromatic rings) content. A very rough protein concentration can be obtained by making the assumption that the protein sample has an extinction coefficient of 1, so 1 OD = 1 mg/ml protein.
by
Abnova
Automated protein synthesizer is an expert system in transcription, translation, and purification of recombinant protein. It is based on the eukaryotic translation apparatus of wheat germ. This high throughput system produces recombinant proteins in vitro. The generated proteins display foldings and biological functions similar to the mammalian counterparts.
by
Abnova
Proximity Ligation Assay is a technology that extends the capabilities of traditional immunofluorescence and immunohistochemistry to include direct detection of proteins, protein interactions and modifications with unparalleled specificity and sensitivity. Target proteins are detected with single molecule resolution, allowing digital quantification and information about the exact localization of the target.
by
Abnova
RNA interference is a gene-silencing technique used in studying the absence of normal gene action. To better understand its function and role in disease, you can transfect siRNA into cell line to turn gene expression off or knock it down.
by
Abnova
The Sandwich ELISA measures the amount of analyte between capture antibody and detection antibody. The analyte needs to have two different epitope sites available for antibody binding.
by
Abnova
SDS-PAGE is a technique used to separate proteins according to their electrophoretic mobility. SDS gel electrophoresis of samples have identical charge per unit mass due to binding of SDS results in fractionation by size, visualize the separated proteins, or process further application.
by
Abnova
Tissue Microarray is a powerful new technology for high throughput analysis of protein expression in a large number of tissue samples. Hundreds of tissue cores are arranged on a single slide, and then analyzed by immunohistochemistry staining. We offer a large collection of immmunohistochmistry (IHC) validated antibodies for the tissue microarray platform.
by
Abnova
Transfected lysate is useful as a positive control in Western Blot. The full-length protein construct is expressed in HEK293T cells and prepared as ready-to-use, denatured lysate in 1x Sample Buffer.
by
Abnova
Transfected lysate is useful as a positive control in Western Blot. The full-length protein construct is expressed in HEK293T cells and prepared as ready-to-use, native lysate in modified RIPA Buffer.
by
Abnova
Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein.
Login
My account
Interest product
Order status
Cart
