Abnova presents Protemist® XE, a fully-automated desktop protein synthesizer for large-scale, low cost, eukaryotic protein production. The system uses a special wheat germ lysate and a batch reaction method developed and prepared by CellFree Sciences Co., Ltd. Protemist® XE is ideally suited for producing 10 mg to over 500 mg of protein for assay development, functional and structural analysis. A unique "Filter-and-Feed" translation method maximizes protein yield by a programmed replenishment of translation buffer and mRNA. The protocol and automation is designed for easy operation obviating the need for manual handling and production.
Fully automated 24- to 48-hour campaign of wheat germ cell-free protein synthesis
Computer-controlled continual filter-and-feed translation through intermittent replenishment of substrate buffer and mRNA
Adjustable translation reaction volume ranging from 10 ml to 50 ml
Approximate 10 mg crude GFP protein production per ml reaction volume with high performance wheat germ extract in WEPRO®7240/8240 series
Principle
Filter-and-Feed Translation Reaction
* Product of Sartorius. The solution is progressively concentrated and micro-molecules pass through
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