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Protemist® DT II - High Throughput Protein Expression
Abnova presents a new generation, fully automated robotic desktop protein synthesizer, Protemist® DT II, for high throughput eykaryotic protein production. The system is developed and manufactured by CellFree Sciences Co., Ltd. It synthesizes the protein of interest using a special wheat germ lysate and a bi-layer reaction. Protemist® DT II can run two scales of translational reaction, 1.2 ml (24 well) or 6 ml (6 well). Batch affinity purification is available for affinity tagged fusion protein synthesized in 6-ml (6 well) reaction scale. This is an expert system in transcription, translation, and purification. The protocol and automation is designed for easy operation obviating the need for manual handling and production.
Wheat germ cell-free protein synthesis
Start with expression vector and gene of interest
Transcription, translation, and purification in less than 35 hours
Batch affinity purification for 6-well format
Approximately 0.5 mg/well (6-well format) or 0.1 mg/well (24-well format) of crude protein production
Wide temperature control range for purification (4°C to 37°C)
Control, record, and data retrieval by PC
Disposable containers, tips, and tubes
Programs for protein synthesis and purification
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1.2-ml translation scale
6-ml translation scale
6-ml scale translation of GST-tagged protein and its purification using reduced glutathione buffer for elution
6-ml scale translation of GST-tagged protein, and its tag removal and purification in the presence of TEV protease
6-ml scale translation of GST-tagged protein, and its tag removal and purification in the presence of PreScission protease
6-ml scale translation of His-tagged protein and its purification using imidazole buffer for elution
6-ml scale translation of His-tagged protein, and its tag removal and purification in the presence of TEV protease
Demonstrations
GST fusion protein expression and purification
Translation (6-ml format) with WEPRO 1240G at 15°C for 20hrs, purification with glutathione-sepharose followed by elution with 1 ml reduction-type glutathione
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