CCNT1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CCNT1 polyclonal antibody (Cat # PAB9999) was diluted 1 : 500 to detect CCNT1 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCNT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human CCNT1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CCNT1 polyclonal antibody (Cat # PAB9999) was diluted 1 : 500 to detect CCNT1 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCNT1
Entrez GeneID
904Gene Name
CCNT1
Gene Alias
CCNT, CYCT1
Gene Description
cyclin T1
Omim ID
602506Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. [provided by RefSeq
Other Designations
CDK9-associated C-type protein|cyclin C-related protein|cyclin T1b|subunit of positive elongation transcription factor b
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Interactome
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Disease
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Publication Reference
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Binding of CDK9 to TRAF2.
MacLachlan TK, Sang N, De Luca A, Puri PL, Levrero M, Giordano A.
Journal of Cellular Biochemistry 1998 Dec; 71(4):467.
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CDK9 (PITALRE): a multifunctional cdc2-related kinase.
de Falco G, Giordano A.
Journal of Cellular Physiology 1998 Dec; 177(4):501.
Application：IP, WB-Ce, WB-Re, WB-Tr, Human, Cancers, Mammalian cells, Recombinant protein.
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Cloning of murine CDK9/PITALRE and its tissue-specific expression in development.
Bagella L, MacLachlan TK, Buono RJ, Pisano MM, Giordano A, De Luca A.
Journal of Cellular Physiology 1998 Nov; 177(2):206.
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Binding of CDK9 to TRAF2.
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