ALOX5 (phospho S523) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of ALOX5 phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the ALOX5 (phospho S523) polyclonal antibody (Cat # PAB9606). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ALOX5.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S523 of human ALOX5.
Host
Rabbit
Theoretical MW (kDa)
80
Reactivity
Human, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of ALOX5 phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the ALOX5 (phospho S523) polyclonal antibody (Cat # PAB9606). The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — ALOX5
Entrez GeneID
240Protein Accession#
P09917Gene Name
ALOX5
Gene Alias
5-LO, 5-LOX, 5LPG, LOG5, MGC163204
Gene Description
arachidonate 5-lipoxygenase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000019502|arachidonic acid 5-lipoxygenase|leukotriene A4 synthase
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523.
Luo M, Jones SM, Phare SM, Coffey MJ, Peters-Golden M, Brock TG.
The Journal of Biological Chemistry 2004 Oct; 279(40):41512.
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Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis.
Dwyer JH, Allayee H, Dwyer KM, Fan J, Wu H, Mar R, Lusis AJ, Mehrabian M.
The New England Journal of Medicine 2004 Jan; 350(1):29.
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Prostaglandins and leukotrienes: advances in eicosanoid biology.
Funk CD.
Science 2001 Nov; 294(5548):1871.
Application：WB, Human, Macrophages, Mast cells, Polymorphonuclear leukocytes.
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Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523.
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