STAT3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
STAT3 polyclonal antibody (Cat # PAB7090) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Tissue lysate)
STAT3 polyclonal antibody (Cat # PAB7090)(0.5 ug/mL) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STAT3 polyclonal antibody (Cat # PAB7090) (5 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry
STAT3 polyclonal antibody (Cat # PAB7090) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STAT3.
Immunogen
A synthetic peptide corresponding to internal region of human STAT3.
Sequence
CRPESQEHPEADP
Host
Goat
Theoretical MW (kDa)
88.1
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Immunohistochemistry (5 ug/mL)
Flow Cytometry (10 ug/mL)
Western Blot (0.5-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STAT3 polyclonal antibody (Cat # PAB7090) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Tissue lysate)
STAT3 polyclonal antibody (Cat # PAB7090)(0.5 ug/mL) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STAT3 polyclonal antibody (Cat # PAB7090) (5 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
STAT3 polyclonal antibody (Cat # PAB7090) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — STAT3
Entrez GeneID
6774Protein Accession#
NP_644805.1;NP_003141.2;NP_998827.1Gene Name
STAT3
Gene Alias
APRF, FLJ20882, HIES, MGC16063
Gene Description
signal transducer and activator of transcription 3 (acute-phase response factor)
Omim ID
102582Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
DNA-binding protein APRF|acute-phase response factor|signal transducer and activator of transcription 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency.
Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD.
Stem Cells 2005 May; 23(5):663.
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Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency.
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