DARC polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
DARC polyclonal antibody (Cat # PAB6873) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DARC polyclonal antibody (Cat # PAB6873) (3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DARC.
Immunogen
A synthetic peptide corresponding to human DARC.
Sequence
HRAELSPSTENSSQLDFED-C
Host
Goat
Theoretical MW (kDa)
35.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DARC polyclonal antibody (Cat # PAB6873) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DARC polyclonal antibody (Cat # PAB6873) (3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — DARC
Entrez GeneID
2532Protein Accession#
NP_002027.2Gene Name
DARC
Gene Alias
CCBP1, CD234, Dfy, FY, GPD, GpFy, WBCQ1
Gene Description
Duffy blood group, chemokine receptor
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
Duffy antigen/chemokine receptor|Duffy blood group antigen|Fy glycoprotein|OTTHUMP00000035293|OTTHUMP00000060081|glycoprotein D
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Interactome
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Disease
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Publication Reference
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Expression of DARC, CXCR3 and CCR5 in giant cell arteritis.
Bruhl H, Vielhauer V, Weiss M, Mack M, Schlondorff D, Segerer S.
Rheumatology (Oxford, England) 2005 Mar; 44(3):309.
Application：IHC-P, Human, Temporal artery.
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Expression of DARC, CXCR3 and CCR5 in giant cell arteritis.
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