GOLM1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GOLM1 polyclonal antibody (Cat # PAB6261)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human GOLM1 with C-terminal MYC tag probed with GOLM1 polyclonal antibody (1 ug/mL) in Lane 1 and probed with anti-MYC Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with GOLM1 polyclonal antibody (1mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GOLM1.
Immunogen
A synthetic peptide corresponding to C-terminus of human GOLM1.
Sequence
C-NLLDQREKRNHTL
Host
Goat
Theoretical MW (kDa)
45.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.2-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GOLM1 polyclonal antibody (Cat # PAB6261)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human GOLM1 with C-terminal MYC tag probed with GOLM1 polyclonal antibody (1 ug/mL) in Lane 1 and probed with anti-MYC Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with GOLM1 polyclonal antibody (1mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GOLM1
Entrez GeneID
51280Protein Accession#
NP_057632.2;NP_808800.1NP_057632Gene Name
GOLM1
Gene Alias
C9orf155, FLJ22634, FLJ23608, GOLPH2, GP73, PSEC0257, bA379P1.3
Gene Description
golgi membrane protein 1
Omim ID
606804Gene Ontology
HyperlinkGene Summary
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000021587|golgi membrane protein GP73|golgi phosphoprotein 2|golgi protein, 73-kD
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Interactome
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Disease
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Publication Reference
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GP73, a novel Golgi-localized protein upregulated by viral infection.
Kladney RD, Bulla GA, Guo L, Mason AL, Tollefson AE, Simon DJ, Koutoubi Z, Fimmel CJ.
Gene 2000 May; 249(1-2):53.
Application：IHC, WB-Ce, WB-Tr, Human, Colon, Kidney, Liver, Lung, Prostate, HEK 293, HepSK-1 cells.
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GP73, a novel Golgi-localized protein upregulated by viral infection.
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