AKR1A1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
AKR1A1 polyclonal antibody (Cat # PAB6212) staining (1 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AKR1A1 polyclonal antibody (Cat # PAB6212) (2.5g/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AKR1A1.
Immunogen
A synthetic peptide corresponding to human AKR1A1.
Sequence
C-DAGHPLYPFNDPY
Host
Goat
Theoretical MW (kDa)
36.6
Reactivity
Human
Specificity
This antibody is expected to recognize both variants (NP_006057.1and NP_697021.1) represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AKR1A1 polyclonal antibody (Cat # PAB6212) staining (1 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AKR1A1 polyclonal antibody (Cat # PAB6212) (2.5g/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKR1A1
Entrez GeneID
10327Protein Accession#
NP_006057.1;NP_697021.1Gene Name
AKR1A1
Gene Alias
ALDR1, ALR, ARM, DD3, MGC12529, MGC1380
Gene Description
aldo-keto reductase family 1, member A1 (aldehyde reductase)
Omim ID
103830Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000009240|OTTHUMP00000009241|alcohol dehydrogenase|aldehyde reductase|aldo-keto reductase family 1, member A1|dihydrodiol dehydrogenase 3
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Interactome
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Pathway
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Disease
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Publication Reference
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The structural organization of the human aldehyde reductase gene, AKR1A1, and mapping to chromosome 1p33-->p32.
Fujii J, Hamaoka R, Matsumoto A, Fujii T, Yamaguchi Y, Egashira M, Miyoshi O, Niikawa N, Taniguchi N.
Cytogenetics and Cell Genetics 1999 Jan; 84(3-4):230.
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The structural organization of the human aldehyde reductase gene, AKR1A1, and mapping to chromosome 1p33-->p32.
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