PDGFRB polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDGFRB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PDGFRB.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDGFRB polyclonal antibody (Cat # PAB3375) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Flow Cytometry
Flow cytometric analysis of SK-BR-3 cells using PDGFRB polyclonal antibody (Cat # PAB3375)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — PDGFRB
Entrez GeneID
5159Protein Accession#
P09619Gene Name
PDGFRB
Gene Alias
CD140B, JTK12, PDGF-R-beta, PDGFR, PDGFR1
Gene Description
platelet-derived growth factor receptor, beta polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq
Other Designations
beta platelet-derived growth factor receptor|platelet-derived growth factor receptor beta|soluble PDGFRb variant 1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Platelet-derived growth factor (PDGF) stimulates glucose transport in 3T3-L1 adipocytes overexpressing PDGF receptor by a pathway independent of insulin receptor substrates.
Whiteman EL, Chen JJ, Birnbaum MJ.
Endocrinology 2003 Sep; 144(9):3811.
Application:WB, Mouse, 3T3-L1 cells.
-
YXXM motifs in the PDGF-beta receptor serve dual roles as phosphoinositide 3-kinase binding motifs and tyrosine-based endocytic sorting signals.
Wu H, Windmiller DA, Wang L, Backer JM.
The Journal of Biological Chemistry 2003 Oct; 278(42):40425.
Application:Func, IP, Human, TRV cells.
-
Beta-platelet-derived growth factor receptor mediates motility and growth of Ewing's sarcoma cells.
Uren A, Merchant MS, Sun CJ, Vitolo MI, Sun Y, Tsokos M, Illei PB, Ladanyi M, Passaniti A, Mackall C, Toretsky JA.
Oncogene 2003 Apr; 22(15):2334.
Application:IHC-P, IP, WB, Human, 5838, 6647, A4573, ES-925, MHH-ES1, RD-ES, TC-32, TC-71, SK-ES cells, Ewing's sarcoma family of tumors.
-
Platelet-derived growth factor (PDGF) stimulates glucose transport in 3T3-L1 adipocytes overexpressing PDGF receptor by a pathway independent of insulin receptor substrates.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com