EPHA3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of EPHA3 polyclonal antibody (Cat # PAB3005) in NCI-H460 (Land 1), 293 (Land 2) cell line lysates (35 ug/lane). EPHA3 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA3 polyclonal antibody (Cat # PAB3005) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHA3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHA3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EPHA3 polyclonal antibody (Cat # PAB3005) in NCI-H460 (Land 1), 293 (Land 2) cell line lysates (35 ug/lane). EPHA3 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA3 polyclonal antibody (Cat # PAB3005) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — EPHA3
Entrez GeneID
2042Protein Accession#
P29320Gene Name
EPHA3
Gene Alias
ETK, ETK1, HEK, HEK4, TYRO4
Gene Description
EPH receptor A3
Omim ID
179611Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq
Other Designations
TYRO4 protein tyrosine kinase|eph-like tyrosine kinase 1|ephrin receptor EphA3|human embryo kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of cell surface targets through meta-analysis of microarray data.
Haeberle H, Dudley JT, Liu JT, Butte AJ, Contag CH.
Neoplasia 2012 Jul; 14(7):666.
Application：IHC-P, Human, Human medulloblastoma, cerebellum.
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Identification of a tumor-specific shared antigen derived from an Eph receptor and presented to CD4 T cells on HLA class II molecules.
Chiari R, Hames G, Stroobant V, Texier C, Maillere B, Boon T, Coulie PG.
Cancer Research 2000 Sep; 60(17):4855.
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Molecular cloning of HEK, the gene encoding a receptor tyrosine kinase expressed by human lymphoid tumor cell lines.
Wicks IP, Wilkinson D, Salvaris E, Boyd AW.
PNAS 1992 Mar; 89(5):1611.
Application：IF, Monkey, COS cells.
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Isolation and characterization of a novel receptor-type protein tyrosine kinase (hek) from a human pre-B cell line.
Boyd AW, Ward LD, Wicks IP, Simpson RJ, Salvaris E, Wilks A, Welch K, Loudovaris M, Rockman S, Busmanis I.
The Journal of Biological Chemistry 1992 Feb; 267(5):3262.
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Identification of cell surface targets through meta-analysis of microarray data.
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