CASP3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CASP3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human CASP3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CASP3 polyclonal antibody (Cat # PAB2069) in NCI-H460 cell line lysates (35 ug/lane).CASP3 (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 polyclonal antibody (Cat # PAB2069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of NCI-H460 cells using CASP3 polyclonal antibody (Cat # PAB2069)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CASP3
Entrez GeneID
836Protein Accession#
NP_004337;P42574Gene Name
CASP3
Gene Alias
CPP32, CPP32B, SCA-1
Gene Description
caspase 3, apoptosis-related cysteine peptidase
Omim ID
600636Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000165054|PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
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Interactome
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Disease
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Publication Reference
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Radiation enhances caspase 3 cleavage of Rad51 in BRCA2-defective cells.
Brown ET, Robinson-Benion C, Holt JT.
Radiation Research 2008 May; 169(5):595.
Application:WB-Ce, Human , Capan-1 cells.
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Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a.
Wright MH, Farquhar MJ, Aletrari MO, Ladds G, Hodgkin MN.
Biochemical and Biophysical Research Communications 2008 May; 369(2):478.
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Differential expression of mycobacterial antigen MPT64, apoptosis and inflammatory markers in multinucleated giant cells and epithelioid cells in granulomas caused by Mycobacterium tuberculosis.
Mustafa T, Wiker HG, Morkve O, Sviland L.
Virchows Archiv: an International Journal of Pathology 2008 Apr; 452(4):449.
Application:IHC-P, Human, Lymph node biopsies from patients with lymphadenitis.
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Radiation enhances caspase 3 cleavage of Rad51 in BRCA2-defective cells.
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