GFAP polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using GFAP polyclonal antibody (Cat # PAB18435).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using GFAP polyclonal antibody (Cat # PAB18435).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using GFAP polyclonal antibody (Cat # PAB18435).
Peptide "+" means "peptide blocking".
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GFAP.
Immunogen
A synthetic peptide corresponding to residues surrounding S38 of human GFAP.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to GFAP.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using GFAP polyclonal antibody (Cat # PAB18435).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using GFAP polyclonal antibody (Cat # PAB18435).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using GFAP polyclonal antibody (Cat # PAB18435).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — GFAP
Entrez GeneID
2670Protein Accession#
P14136Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
-
-
Interactome
-
Disease
-
Publication Reference
-
Effects of NOTCH1 signaling inhibitor γ-secretase inhibitor II on growth of cancer stem cells.
Ding X, Ding C, Wang F, Deng W, Yu M, Meng Q, Sun P.
Oncology Letters 2018 Nov; 16(5):6095.
Application：IF, Human, U87 MG cells.
-
Effects of NOTCH1 signaling inhibitor γ-secretase inhibitor II on growth of cancer stem cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com