BRAF polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BRAF.
Immunogen
A synthetic peptide corresponding to residues surrounding S601 of human BRAF.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to BRAF.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells, using BRAF polyclonal antibody (Cat # PAB18274).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lymph node tissue using BRAF polyclonal antibody (Cat # PAB18274).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRAF
Entrez GeneID
673Protein Accession#
P15056Gene Name
BRAF
Gene Alias
B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
Other Designations
94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|Murine sarcoma viral (v-raf) oncogene homolog B1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Mutation of B-Raf in human choroidal melanoma cells mediates cell proliferation and transformation through the MEK/ERK pathway.
Calipel A, Lefevre G, Pouponnot C, Mouriaux F, Eychene A, Mascarelli F.
J Biol Chem 2003 Oct; 278(43):42409.
Application:IP-WB, WB-Tr, Human, NCM, OCM-1 cells.
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Ectopic B-Raf expression enhances extracellular signal-regulated kinase (ERK) signaling in T cells and prevents antigen-presenting cell-induced anergy.
Dillon TJ, Karpitski V, Wetzel SA, Parker DC, Shaw AS, Stork PJ.
J Biol Chem 2003 Sep; 278(38):35940.
Application:Flow Cyt, WB-ce, Mouse, Thymocytes, T cell blasts.
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Mutation of B-Raf in human choroidal melanoma cells mediates cell proliferation and transformation through the MEK/ERK pathway.
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