BMI1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The BMI1 polyclonal antibody (Cat # PAB1687) is used in Western blot to detect BMI1 in mouse muscle tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BMI1 polyclonal antibody (Cat # PAB1687) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BMI1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human BMI1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The BMI1 polyclonal antibody (Cat # PAB1687) is used in Western blot to detect BMI1 in mouse muscle tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BMI1 polyclonal antibody (Cat # PAB1687) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — BMI1
Entrez GeneID
648Protein Accession#
NP_005171;P35226Gene Name
BMI1
Gene Alias
MGC12685, PCGF4, RNF51
Gene Description
BMI1 polycomb ring finger oncogene
Omim ID
164831Gene Ontology
HyperlinkOther Designations
B lymphoma Mo-MLV insertion region 1 homolog|flvi-2/bmi-1|murine leukemia viral (bmi-1) oncogene homolog|oncogene BMI-1|polycomb group ring finger 4
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Interactome
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Publication Reference
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Bmi-1 regulation of INK4A-ARF is a downstream requirement for transformation of hematopoietic progenitors by E2a-Pbx1.
Smith KS, Chanda SK, Lingbeek M, Ross DT, Botstein D, van Lohuizen M, Cleary ML.
Molecular Cell 2003 Aug; 12(2):393.
Application：WB, Human, Human leukemia cell lines, including 697, Hal-01, HB1119, RCH-ACV, Reh, RS4:11 cells; Human pre-B cell line A2.
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MLL repression domain interacts with histone deacetylases, the polycomb group proteins HPC2 and BMI-1, and the corepressor C-terminal-binding protein.
Xia ZB, Anderson M, Diaz MO, Zeleznik-Le NJ.
PNAS 2003 Jul; 100(14):8342.
Application：WB-Tr, Human, HEK 293T cells.
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Control of the replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1.
Itahana K, Zou Y, Itahana Y, Martinez JL, Beausejour C, Jacobs JJ, Van Lohuizen M, Band V, Campisi J, Dimri GP.
Molecular and Cellular Biology 2003 Jan; 23(1):389.
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Bmi-1 regulation of INK4A-ARF is a downstream requirement for transformation of hematopoietic progenitors by E2a-Pbx1.
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