FAS polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FAS.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human FAS.
Sequence
KTCRKHRKENQGSH
Host
Goat
Theoretical MW (kDa)
37.7, 35.4, 24.8, 16
Reactivity
Human
Specificity
This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
FAS polyclonal antibody (Cat # PAB16642) (0.1 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FAS
Entrez GeneID
355Protein Accession#
NP_000034.1;NP_690610.1;NP_690611.1Gene Name
FAS
Gene Alias
ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6
Gene Description
Fas (TNF receptor superfamily, member 6)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq
Other Designations
APO-1 cell surface antigen|CD95 antigen|Fas AMA|Fas antigen|OTTHUMP00000020045|OTTHUMP00000020046|OTTHUMP00000020051|OTTHUMP00000059646|apoptosis antigen 1|tumor necrosis factor receptor superfamily member 6|tumor necrosis factor receptor superfamily, mem
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Interactome
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Pathway
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Disease
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Publication Reference
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Reduced FAS transcription in clones of U937 cells that have acquired resistance to Fas-induced apoptosis.
Blomberg J, Ruuth K, Jacobsson M, Hoglund A, Nilsson JA, Lundgren E.
FEBS Letters 2009 Jan; 276(2):497.
Application:Func, Human , U-937 cells.
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Reduced FAS transcription in clones of U937 cells that have acquired resistance to Fas-induced apoptosis.
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