GAPDH polyclonal antibody
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More Files
- More Functions
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GAPDH.
Immunogen
A synthetic peptide corresponding to C-terminus 16 amino acids of human GAPDH.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of GAPDH in HeLa cell lysate with GAPDH polyclonal antibody (Cat # PAB13195) at (A) 0.5, (B) 1 and (C) 2 ug/mL .Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of GAPDH in HeLa cells at a dilution of 10 ug/mL . -
Gene Info — GAPDH
Entrez GeneID
2597Protein Accession#
P04406Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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Publication Reference
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Antitumor Activity of Amivantamab (JNJ-61186372), an EGFR-MET Bispecific Antibody, in Diverse Models of EGFR Exon 20 Insertion-Driven NSCLC.
Jiyeon Yun, Soo-Hwan Lee, Seok-Young Kim, Seo-Yoon Jeong, Jae-Hwan Kim, Kyoung-Ho Pyo, Chae-Won Park, Seong Gu Heo, Mi Ran Yun, Sangbin Lim, Sun Min Lim, Min Hee Hong, Hye Ryun Kim, Meena Thayu, Joshua C Curtin, Roland E Knoblauch, Matthew V Lorenzi, Amy Roshak, Byoung Chul Cho
Cancer Discovery 2020 May; 10(8):1194.
Application:WB, Human, Mouse, Ba/F3, Mouse tumor, YU-1163 cells.
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Dried plum and chokeberry ameliorate d-galactose-induced aging in mice by regulation of Pl3k/Akt-mediated Nrf2 and Nf-kB pathways.
Jeong H, Liu Y, Kim HS.
Experimental Gerontology 2017 May; 95:16.
Application:WB-Ti, Mouse, Mouse liver.
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New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells.
Sirover MA.
Journal of Cellular Biochemistry 2005 May; 95(1):45.
Application:IHC, WB, Human, Mouse, Mammalian cells.
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Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases.
Chuang DM, Hough C, Senatorov VV.
Annual Review of Pharmacology and Toxicology 2005 Jan; 45:269.
Application:IF, IHC, Mouse, Mouse brains.
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Antitumor Activity of Amivantamab (JNJ-61186372), an EGFR-MET Bispecific Antibody, in Diverse Models of EGFR Exon 20 Insertion-Driven NSCLC.
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