KDR polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue was stained with KDR polyclonal antibody (Cat # PAB12647) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Immunoprecipitation
The whole cell lysate derived from human umbilical vein endothelial cell (HUVEC) was stimulated by VEGF-A for 20 min, then immunoprecipitated by KDR polyclonal antibody (Cat # PAB12647) followed by immune-probing with KDR polyclonal antibody (Cat # PAB12647) at 1 : 500. The immunoreactive bands are observed around ~ 200 KDa (non-phospho form) and ~ 240 KDa (Phospho-form, lane 1). The lane 2 is a negative control.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KDR.
Immunogen
A synthetic peptide corresponding to extracellular domain of human KDR.
Host
Rabbit
Theoretical MW (kDa)
200, 240
Reactivity
Human, Mouse
Specificity
This antibody recognizes ~200 and ~240 KDa of human KDR.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue was stained with KDR polyclonal antibody (Cat # PAB12647) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
The whole cell lysate derived from human umbilical vein endothelial cell (HUVEC) was stimulated by VEGF-A for 20 min, then immunoprecipitated by KDR polyclonal antibody (Cat # PAB12647) followed by immune-probing with KDR polyclonal antibody (Cat # PAB12647) at 1 : 500. The immunoreactive bands are observed around ~ 200 KDa (non-phospho form) and ~ 240 KDa (Phospho-form, lane 1). The lane 2 is a negative control.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KDR
Entrez GeneID
3791Gene Name
KDR
Gene Alias
CD309, FLK1, VEGFR, VEGFR2
Gene Description
kinase insert domain receptor (a type III receptor tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq
Other Designations
soluble VEGFR2|vascular endothelial growth factor receptor 2
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Alzheimer disease
- Angina Pectoris
- Anoxia
- Asthma
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
+ View More Disease
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Publication Reference
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Soluble vascular endothelial growth factor receptor 3 is essential for corneal alymphaticity.
Singh N, Tiem M, Watkins R, Cho YK, Wang Y, Olsen T, Uehara H, Mamalis C, Luo L, Oakey Z, Ambati BK.
Blood 2013 May; 121(20):4242.
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY.
Molecular & Cellular Proteomics 2013 May; 12(5):1335.
Application：Profiling, Human, Huh7 cells, Mahlavu cells.
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VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis.
Matsumoto T, Bohman S, Dixelius J, Berge T, Dimberg A, Magnusson P, Wang L, Wikner C, Qi JH, Wernstedt C, Wu J, Bruheim S, Mugishima H, Mukhopadhyay D, Spurkland A, Claesson-Welsh L.
The EMBO Journal 2005 Jul; 24(13):2342.
Application：IF, WB-Tr, Human, Pig, HUVECs, PAE cells.
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Soluble vascular endothelial growth factor receptor 3 is essential for corneal alymphaticity.
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