CHUK (Human) Recombinant Protein
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Specification
Product Description
Human CHUK (NP_001269.3, 1 a.a. - 745 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
111
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
93 % by SDS-PAGE/CBB staining
Activity
The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization). Substrate: I-kappaB-alpha peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CHUK
Entrez GeneID
1147Protein Accession#
NP_001269.3Gene Name
CHUK
Gene Alias
IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Gene Description
conserved helix-loop-helix ubiquitous kinase
Omim ID
600664Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq
Other Designations
I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|OTTHUMP00000020273|conserved helix-loop ubiquitous kinase
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