GAPDH monoclonal antibody, clone AT8G4
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Anti-human GAPDH antibody (1:1000), Lane 2: Anti-human GAPDH antibody (1:2000), Lane 3: Anti-human GAPDH antibody (1:4000), Lane 4: Anti-human GAPDH antibody (1:6000), Lane 5: Anti-human GAPDH antibody (1:8000), Lane 6: Anti-human GAPDH antibody (1:10000).
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 500) with Texas Red (Red).
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GAPDH.
Immunogen
Recombinant protein corresponding to full length human GAPDH.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: Anti-human GAPDH antibody (1:1000), Lane 2: Anti-human GAPDH antibody (1:2000), Lane 3: Anti-human GAPDH antibody (1:4000), Lane 4: Anti-human GAPDH antibody (1:6000), Lane 5: Anti-human GAPDH antibody (1:8000), Lane 6: Anti-human GAPDH antibody (1:10000).Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 500) with Texas Red (Red).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — GAPDH
Entrez GeneID
2597Protein Accession#
NP_002037Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
-
Interactome
-
Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
-
Disease
-
Publication Reference
-
Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases.
Chuang DM, Hough C, Senatorov VV.
Annual Review of Pharmacology and Toxicology 2005 Jan; 45:269.
Application：IF, IHC, Mouse, Mouse brains.
-
Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders?
Mazzola JL, Sirover MA.
Neurotoxicology 2002 Oct; 23(4-5):603.
-
Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com