ITGB2 monoclonal antibody, clone IB4
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More Files
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Flow Cytometry
5 X 105 cultured human HPB-MLT cells per tube were incubated 45 minutes on ice with 80 uL of ITGB2 monoclonal antibody, clone IB4 (Cat # MAB6945) at a concentration of 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive (67% net) with a mean shift of 0.92 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
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Specification
Product Description
Mouse monoclonal antibody raised against native ITGB2.
Immunogen
Native purified ITGB2 from human peripheral blood monocytes.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 cultured human HPB-MLT cells per tube were incubated 45 minutes on ice with 80 uL of ITGB2 monoclonal antibody, clone IB4 (Cat # MAB6945) at a concentration of 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive (67% net) with a mean shift of 0.92 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. -
Gene Info — ITGB2
Entrez GeneID
3689Gene Name
ITGB2
Gene Alias
CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7
Gene Description
integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
CD18 subunit|OTTHUMP00000115278|OTTHUMP00000115279|OTTHUMP00000115281|OTTHUMP00000115282|beta 2 integrin subunit|cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor)|complement receptor C3 beta-subunit|integrin beta 2|integrin bet
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Interactome
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Pathway
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Disease
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Publication Reference
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A crucial role for beta 2 integrin in the activation of eosinophils stimulated by IgG.
Kaneko M, Horie S, Kato M, Gleich GJ, Kita H.
Journal of Immunology 1995 Sep; 155(5):2631.
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Leukocyte CD11/CD18 integrins: biological and clinical relevance.
Mazzone A, Ricevuti G.
Haematologica 1995 Mar; 80(2):161.
Application：Flow Cyt, Neutralization, Human, Mouse, B cells, Erythroid cells, Macrophages, Monocytes, Myeloid cells, T cells.
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Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.
Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N.
The Journal of Cell Biology 1994 Jul; 126(2):529.
Application：Func, Human, Human T cells.
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A crucial role for beta 2 integrin in the activation of eosinophils stimulated by IgG.
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