MDM4 (phospho S367) monoclonal antibody, clone #15
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More Files
- More Functions
Western Blot (Cell lysate)
Induction of S367 phosphorylation after DNA damage is associated with increased binding of 14-3-3 to MDM4 and accelerated MDM4 degradation.
MCF-7 cells were preincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM), for the indicated periods.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of MDM4.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S367 of human MDM4.
Host
Mouse
Reactivity
Human, Mouse
Specificity
This antibody reacts with MDM4 phosphorylated at Ser367.
Form
Liquid
Isotype
IgG2b, kappa
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Induction of S367 phosphorylation after DNA damage is associated with increased binding of 14-3-3 to MDM4 and accelerated MDM4 degradation.
MCF-7 cells were preincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM), for the indicated periods.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MDM4
Entrez GeneID
4194Gene Name
MDM4
Gene Alias
DKFZp781B1423, HDMX, MDMX, MGC132766, MRP1
Gene Description
Mdm4 p53 binding protein homolog (mouse)
Omim ID
602704Gene Ontology
HyperlinkGene Summary
The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq
Other Designations
MDM4-related protein 1|Mdm4, transformed 3T3 cell double minute 4, p53 binding protein|double minute 4, human homolog of; p53-binding protein|mouse double minute 4 homolog|p53-binding protein
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Interactome
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Pathway
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Disease
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