FADD monoclonal antibody, clone J1D2
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: A431 cell lysate, Lane 5: Ramos cell lysate, Lane 6: Raji cell lysate, Lane 7: Balb/3T3 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast cancer tissue were incubated with FADD monoclonal antibody, clone J1D2 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with FADD monoclonal antibody, clone J1D2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FADD.
Immunogen
Recombinant protein corresponding to amino acids 1-208 of human FADD.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: A431 cell lysate, Lane 5: Ramos cell lysate, Lane 6: Raji cell lysate, Lane 7: Balb/3T3 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast cancer tissue were incubated with FADD monoclonal antibody, clone J1D2 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with FADD monoclonal antibody, clone J1D2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FADD
Entrez GeneID
8772Protein Accession#
NP_003815Gene Name
FADD
Gene Alias
GIG3, MGC8528, MORT1
Gene Description
Fas (TNFRSF6)-associated via death domain
Omim ID
602457Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq
Other Designations
Fas-associated via death domain|Fas-associating death domain-containing protein|Fas-associating protein with death domain|growth-inhibiting gene 3 protein|mediator of receptor-induced toxicity
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Interactome
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Pathway
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Disease
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Publication Reference
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Japanese encephalitis virus infection activates caspase-8 and -9 in a FADD-independent and mitochondrion-dependent manner.
Tsao CH, Su HL, Lin YL, Yu HP, Kuo SM, Shen CI, Chen CW, Liao CL.
The Journal of General Virology 2008 Aug; 89(Pt 8):1930.
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FADD adaptor in cancer.
Tourneur L, Buzyn A, Chiocchia G.
Medical Immunology (London, England) 2005 Feb; 4(1):1.
Application：IHC-P, WB-Tr, Human, Mouse, Tumors.
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The Fas-associated death domain protein suppresses activation of NF-kappa B by LPS and IL-1 beta.
Bannerman DD, Tupper JC, Kelly JD, Winn RK, Harlan JM.
The Journal of Clinical Investigation 2002 Feb; 109(3):419.
Application：WB-Tr, Human, Mouse, HMEC-1 cells, MEFs.
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Japanese encephalitis virus infection activates caspase-8 and -9 in a FADD-independent and mitochondrion-dependent manner.
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