PDCD1 monoclonal antibody, clone 4F12
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More Files
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Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with PDCD1 monoclonal antibody, clone 4F12 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x106cellscells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PDCD1.
Immunogen
Recombinant protein corresponding to amino acids 21-167 of human PDCD1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with PDCD1 monoclonal antibody, clone 4F12 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x106cellscells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — PDCD1
Entrez GeneID
5133GeneBank Accession#
BC074740Protein Accession#
AAH74740Gene Name
PDCD1
Gene Alias
CD279, PD1, SLEB2, hPD-1, hPD-l
Gene Description
programmed cell death 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Immunohistochemical analysis and prognostic significance of PD-L1, PD-1, and CD8+ tumor-infiltrating lymphocytes in Ewing's sarcoma family of tumors (ESFT).
Machado I, López-Guerrero JA, Scotlandi K, Picci P, Llombart-Bosch A.
Virchows Archiv : an International Journal of Pathology 2018 May; 472(5):815.
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PD-1 immunoreceptor inhibits B cell receptor-mediated signaling by recruiting src homology 2-domain-containing tyrosine phosphatase 2 to phosphotyrosine.
Okazaki T, Maeda A, Nishimura H, Kurosaki T, Honjo T.
PNAS 2001 Nov; 98(24):13866.
Application：Flow Cyt, Func, Mouse, Mouse A20 B cell lymphoma IIA1.6.
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PD-1: an inhibitory immunoreceptor involved in peripheral tolerance.
Nishimura H, Honjo T.
Trends in Immunology 2001 May; 22(5):265.
Application：WB, Human, Human thymocytes.
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PD-L2 is a second ligand for PD-1 and inhibits T cell activation.
Latchman Y, Wood CR, Chernova T, Chaudhary D, Borde M, Chernova I, Iwai Y, Long AJ, Brown JA, Nunes R, Greenfield EA, Bourque K, Boussiotis VA, Carter LL, Carreno BM, Malenkovich N, Nishimura H, Okazaki T, Honjo T, Sharpe AH, Freeman GJ.
Nature Immunology 2001 Mar; 2(3):261.
Application：Flow Cyt, Mouse, CHO cells.
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Immunohistochemical analysis and prognostic significance of PD-L1, PD-1, and CD8+ tumor-infiltrating lymphocytes in Ewing's sarcoma family of tumors (ESFT).
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