IL1B monoclonal antibody, clone 3A6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) against human IL1B (aa 126-261) recombinant protein. (Expected MW is 41 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human muscle (upper) and human lung cancer (bottom) tissues using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HepG2 cells using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) .
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant IL1B.
Immunogen
Recombinant protein corresponding to human IL1B.
Host
Mouse
Theoretical MW (kDa)
31
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) against human IL1B (aa 126-261) recombinant protein. (Expected MW is 41 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human muscle (upper) and human lung cancer (bottom) tissues using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HepG2 cells using IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of IL1B monoclonal antibody, clone 3A6 (Cat # MAB10697) . -
Gene Info — IL1B
Entrez GeneID
3553Gene Name
IL1B
Gene Alias
IL-1, IL1-BETA, IL1F2
Gene Description
interleukin 1, beta
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq
Other Designations
catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
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Interactome
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