BMI1 monoclonal antibody, clone 3E3
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) against human BMI1 (aa : 1-326) recombinant protein. (Expected MW is 62.4 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human rectum cancer (B) tissues using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) (green) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BMI1.
Immunogen
Recombinant protein corresponding to human BMI1.
Host
Mouse
Theoretical MW (kDa)
37
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot analysis using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) against human BMI1 (aa : 1-326) recombinant protein. (Expected MW is 62.4 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) and human rectum cancer (B) tissues using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506).Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using BMI1 monoclonal antibody, clone 3E3 (Cat # MAB10506) (green) and negative control (red). -
Gene Info — BMI1
Entrez GeneID
648Gene Name
BMI1
Gene Alias
MGC12685, PCGF4, RNF51
Gene Description
BMI1 polycomb ring finger oncogene
Omim ID
164831Gene Ontology
HyperlinkOther Designations
B lymphoma Mo-MLV insertion region 1 homolog|flvi-2/bmi-1|murine leukemia viral (bmi-1) oncogene homolog|oncogene BMI-1|polycomb group ring finger 4
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com