OTX2 monoclonal antibody, clone 1H12G8B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) against recombinant human OTX2 protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon tissue (A), colon cancer tissue (B), stomach tissue (C) and brain tissue (D) using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of U251 cells using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant OTX2.
Immunogen
Recombinant protein corresponding to human OTX2.
Host
Mouse
Theoretical MW (kDa)
32
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) against recombinant human OTX2 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon tissue (A), colon cancer tissue (B), stomach tissue (C) and brain tissue (D) using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) with DAB staining.Immunofluorescence
Immunofluorescence analysis of U251 cells using OTX2 monoclonal antobody, clone 1H12G8B2 (Cat # MAB10289) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — OTX2
Entrez GeneID
5015Gene Name
OTX2
Gene Alias
MCOPS5, MGC45000
Gene Description
orthodenticle homeobox 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000064776|homeobox protein OTX2|orthodenticle homolog 2
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Interactome
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Disease
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