CD63 monoclonal antibody, clone MEM-259
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More Files
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Immunocytochemistry
Immunofluorescence staining of (A) human skin fibroblasts and (B) human primary fibroblasts with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green) after co-incubation of living cells with human Transferrin-Dyomics 547 (red) ; cell nuclei stained with DAPI (blue) .
Immunocytochemistry
Immunofluorescence staining of CD63 in human HeLa cell line using CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
Flow Cytometry
Flow cytometry analysis of IgE-activated human peripheral blood stained with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931).
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Specification
Product Description
Mouse monoclonal antibody raised against native CD63.
Immunogen
Native purified CD63 from human T cell line HPB-ALL.
Host
Mouse
Reactivity
Human
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence staining of (A) human skin fibroblasts and (B) human primary fibroblasts with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green) after co-incubation of living cells with human Transferrin-Dyomics 547 (red) ; cell nuclei stained with DAPI (blue) .Immunocytochemistry
Immunofluorescence staining of CD63 in human HeLa cell line using CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931, green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .Immunoprecipitation
Flow Cytometry
Flow cytometry analysis of IgE-activated human peripheral blood stained with CD63 monoclonal antibody, clone MEM-259 (Cat # MAB0931). -
Gene Info — CD63
Entrez GeneID
967Gene Name
CD63
Gene Alias
LAMP-3, ME491, MLA1, OMA81H, TSPAN30
Gene Description
CD63 molecule
Omim ID
155740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq
Other Designations
CD63 antigen|CD63 antigen (melanoma 1 antigen)|granulophysin|lysosome-associated membrane glycoprotein 3|melanoma 1 antigen|melanoma-associated antigen ME491|melanoma-associated antigen MLA1|ocular melanoma-associated antigen|tetraspanin-30
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Interactome
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Pathway
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Publication Reference
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Ultrasonics-Assisted Effective Isolation and Characterization of Exosomes from Whole Organs.
Burak Derkus, Emel Emregul.
Methods in Molecular Biology (Clifton, N.J.) 2021 Jan; 2207:25.
Application：WB-Ti, Mouse, Mouse brain, Mouse heart, Mouse liver.
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The Role of Transforming Growth Factor β in Cell-to-Cell Contact-Mediated Epstein-Barr Virus Transmission.
Nanbo A, Ohashi M, Yoshiyama H, Ohba Y.
Frontiers in Microbiology 2018 May; 9:984.
Application：WB-Ce, Human, Exosomes released from Akata Cells.
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Ultrasonics-Assisted Effective Isolation and Characterization of Exosomes from Whole Organs.
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