ANGPTL5 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human ANGPTL5 full-length ORF ( AAH49170.1, 1 a.a. - 388 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MMSPSQASLLFLNVCIFICGEAVQGNCVHHSTDSSVVNIVEDGSNAKDESKSNDTVCKEDCEESCDVKTKITREEKHFMCRNLQNSIVSYTRSTKKLLRNMMDEQQASLDYLSNQVNELMNRVLLLTTEVFRKQLDPFPHRPVQSHGLDCTDIKDTIGSVTKTPSGLYIIHPEGSSYPFEVMCDMDYRGGGWTVIQKRIDGIIDFQRLWCDYLDGFGDLLGEFWLGLKKIFYIVNQKNTSFMLYVALESEDDTLAYASYDNFWLEDETRFFKMHLGRYSGNAGDAFRGLKREDNQNAKPFSTSDVDNDGCRPACLVNGQSVKSCSHLHNKTGWWFNECGLANLNGIHHFSGKLLATGIQWGTWTKNNSPVKIKSVSMKIRRMYNPYFK
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
68.2
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ANGPTL5
Entrez GeneID
253935GeneBank Accession#
BC049170Protein Accession#
AAH49170.1Gene Name
ANGPTL5
Gene Alias
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Gene Description
angiopoietin-like 5
Omim ID
607666Gene Ontology
HyperlinkOther Designations
fibrinogen-like
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Publication Reference
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Human CD34 CD133 Hematopoietic Stem Cells Cultured with Growth Factors Including Angptl5 Efficiently Engraft Adult NOD-SCID Il2r{gamma} (NSG) Mice.
Drake AC, Khoury M, Leskov I, Iliopoulou BP, Fragoso M, Lodish H, Chen J.
PLoS One 2011 Apr; 6(4):e18382.
Application:Func , Human, Hematopoietic stem cells.
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Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem and progenitor cells.
Walenda T, Bokermann G, Ferreira MV, Piroth DM, Hieronymus T, Neuss S, Zenke M, Ho AD, Muller AM, Wagner W.
Exp Hematol 2011 Feb; 39:617.
Application:Incubated, Recombinant protein.
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Densely interconnected transcriptional circuits control cell states in human hematopoiesis.
Noa Novershtern, Aravind Subramanian, Lee N Lawton, Raymond H Mak, W Nicholas Haining, Marie E McConkey, Naomi Habib, Nir Yosef, Cindy Y Chang, Tal Shay, Garrett M Frampton, Adam C B Drake, Ilya Leskov, Bjorn Nilsson, Fred Preffer, David Dombkowski, John W Evans, Ted Liefeld, John S Smutko, Jianzhu Chen, Nir Friedman, Richard A Young, Todd R Golub, Aviv Regev, Benjamin L Ebert.
Cell 2011 Jan; 144(2):296.
Application:Sub, Human, Human Hematopoietic Stem cell.
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IGFBP2 Supports ex vivo Expansion of Hematopoietic Stem Cells.
Huynh H, Kaba M, Rudra S, Zheng J, Wu CJ, Lodish HF, Zhang CC.
Springer, Berlin, Heidelberg 2009 Nov; 21.
Application:Func, Human, Human cord blood cells.
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Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation.
Zhang CC, Kaba M, Iizuka S, Huynh H, Lodish HF.
Blood 2008 Jan; 111(7):3415.
Application:Func, Human, Human cord blood mononuclear cells.
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Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells.
Zhang CC, Kaba M, Ge G, Xie K, Tong W, Hug C, Lodish HF.
Nature Medicine 2006 Jan; 12(2):240.
Application:SDS-PAGE, Mouse, Mouse bone marrow SP Sca-1+ CD45+ cells.
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Human CD34 CD133 Hematopoietic Stem Cells Cultured with Growth Factors Including Angptl5 Efficiently Engraft Adult NOD-SCID Il2r{gamma} (NSG) Mice.
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