AADAT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
AADAT transfected lysate.
Western Blot
Lane 1: AADAT transfected lysate ( 47.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AADAT full-length
Host
Human
Theoretical MW (kDa)
47.3
Interspecies Antigen Sequence
Mouse (72); Rat (71)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AADAT antibody (H00051166-B01) by Western Blots.
SDS-PAGE Gel
AADAT transfected lysate.
Western Blot
Lane 1: AADAT transfected lysate ( 47.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AADAT
Entrez GeneID
51166GeneBank Accession#
BC031068.1Protein Accession#
AAH31068.1Gene Name
AADAT
Gene Alias
KAT2, KATII
Gene Description
aminoadipate aminotransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist. [provided by RefSeq
Other Designations
L kynurenine/alpha aminoadipate aminotransferase|L-kynurenine/alpha-aminoadipate aminotransferase|alpha-aminoadipate aminotransferase|kynurenine aminotransferase II
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Interactome
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Pathway
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