PSCD3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PSCD3 transfected lysate.
Western Blot
Lane 1: PSCD3 transfected lysate ( 44 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSCD3 full-length
Host
Human
Theoretical MW (kDa)
44
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSCD3 antibody (H00009265-B01) by Western Blots.
SDS-PAGE Gel
PSCD3 transfected lysate.
Western Blot
Lane 1: PSCD3 transfected lysate ( 44 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYTH3
Entrez GeneID
9265GeneBank Accession#
NM_004227.3Protein Accession#
NP_004218.1Gene Name
CYTH3
Gene Alias
ARNO3, GRP1, PSCD3
Gene Description
cytohesin 3
Omim ID
605081Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function, and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions, in addition to acting on ARF1. [provided by RefSeq
Other Designations
ARF nucleotide-binding site opener 3|general receptor of phosphoinositides 1|pleckstrin homology, Sec7 and coiled-coil domains 3|pleckstrin homology, Sec7 and coiled/coil domains 3
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