CLDN1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CLDN1 transfected lysate.
Western Blot
Lane 1: CLDN1 transfected lysate ( 22.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CLDN1 full-length
Host
Human
Theoretical MW (kDa)
22.7
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CLDN1 antibody (H00009076-D01P) by Western Blots.
SDS-PAGE Gel
CLDN1 transfected lysate.
Western Blot
Lane 1: CLDN1 transfected lysate ( 22.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CLDN1
Entrez GeneID
9076GeneBank Accession#
NM_021101.3Protein Accession#
NP_066924.1Gene Name
CLDN1
Gene Alias
CLD1, ILVASC, SEMP1
Gene Description
claudin 1
Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq
Other Designations
senescence-associated epithelial membrane protein 1
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Interactome
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Pathway
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Disease
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