CLDN1 monoclonal antibody (M02), clone 2E2-H5
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M02), clone 2E2-H5.
Lane 1: CLDN1 transfected lysate (Predicted MW: 22.7 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CLDN1 is 0.3 ng/ml as a capture antibody.
Immunofluorescence (Circulating Tumor Cell)
Huh7 cells were stained with CLDN1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to CLDN1 on Huh7 cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (48.95 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CLDN1.
Immunogen
CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (48.95 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M02), clone 2E2-H5.
Lane 1: CLDN1 transfected lysate (Predicted MW: 22.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CLDN1 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence (Circulating Tumor Cell)
Huh7 cells were stained with CLDN1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to CLDN1 on Huh7 cell. [antibody concentration 10 ug/ml] -
Gene Info — CLDN1
Entrez GeneID
9076GeneBank Accession#
BC012471Protein Accession#
AAH12471.1Gene Name
CLDN1
Gene Alias
CLD1, ILVASC, SEMP1
Gene Description
claudin 1
Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq
Other Designations
senescence-associated epithelial membrane protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures.
Beáta Szilvia Bolla, Lilla Erdei, Edit Urbán, Katalin Burián, Lajos Kemény, Kornélia Szabó.
Scientific Reports 2020 Jul; 10(1):12815.
Application：WB-Ce, Human, Human keratinocytes.
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Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures.
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