KRAS monoclonal antibody (M01), clone 3B10-2F2
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More Files
- More Functions
Western Blot (Cell lysate)
KRAS monoclonal antibody (M01), clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.
Lane 1: KRAS transfected lysate(21 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRAS on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (46.42 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant KRAS.
Immunogen
KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.42 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
KRAS monoclonal antibody (M01), clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.
Lane 1: KRAS transfected lysate(21 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRAS on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — KRAS
Entrez GeneID
3845GeneBank Accession#
BC013572Protein Accession#
AAH13572Gene Name
KRAS
Gene Alias
C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2
Gene Description
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Ontology
HyperlinkGene Summary
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq
Other Designations
K-ras p21 protein|Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog|PR310 c-K-ras oncogene|c-K-ras2 protein|c-Kirsten-ras protein|cellular c-Ki-ras2 proto-oncogene|oncogene KRAS2|transforming protein p21|v-Ki-ras2 Kirsten rat sarcoma 2 viral oncoge
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Interactome
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Pathway
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Disease
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Publication Reference
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Oncogenic KRAS mutation confers chemoresistance by upregulating SIRT1 in non-small cell lung cancer.
Dong Hoon Shin, Jeong Yeon Jo, Minyoung Choi, Kyung-Hee Kim, Young-Ki Bae, Sang Soo Kim.
Experimental & Molecular Medicine 2023 Oct; 55(10):2220.
Application：WB, Human, Mouse, HEK293T cells, Lung.
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Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi.
Alexandra M Amen, Ryan M Loughran, Chun-Hao Huang, Rachel J Lew, Archna Ravi, Yuanzhe Guan, Emma M Schatoff, Lukas E Dow, Brooke M Emerling, Christof Fellmann.
Cell Reports Methods 2022 Jun; 2(7):100239.
Application：WB-Tr, Human, H1975, PC9 cells.
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EndoBind detects endogenous protein-protein interactions in real time.
Anke Bill, Sheryll Espinola, Daniel Guthy, Jacob R Haling, Mylene Lanter, Min Lu, Anthony Marelli, Angelica Mendiola, Loren Miraglia, Brandon L Taylor, Leonardo Vargas, Anthony P Orth, Frederick J King.
Communications Biology 2021 Sep; 4(1):1085.
Application：WB-Tr, Human, PATU8988T cells.
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The Combination of Loss of ALDH1L1 Function and Phenformin Treatment Decreases Tumor Growth in KRAS-Driven Lung Cancer.
Seon-Hyeong Lee, Yoon Jeon, Joon Hee Kang, Hyonchol Jang, Ho Lee, Soo-Youl Kim.
Cancers 2020 May; 12(6):E1382.
Application：WB-Tr, Human, A-549 cells, H1299 cells, H1975 cells, H23 cells, H460 cells, HOP-62 cells .
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Grifolin, neogrifolin and confluentin from the terricolous polypore Albatrellus flettii suppress KRAS expression in human colon cancer cells.
Almas Yaqoob, Wai Ming Li, Victor Liu, Chuyi Wang, Sebastian Mackedenski, Linda E Tackaberry, Hugues B Massicotte, Keith N Egger, Kerry Reimer, Chow H Lee.
PLoS One 2020 May; 15(5):e0231948.
Application：WB-Ce, Human, SW480 cells.
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K-Ras G-domain binding with signaling lipid phosphatidylinositol (4,5)-phosphate (PIP2): membrane association, protein orientation, and function.
Cao S, Chung S, Kim S, Li Z, Manor D, Buck M.
The Journal of Biological Chemistry 2019 Apr; 294(17):7068.
Application：IF, Mouse, NIH/3T3 cells.
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Bilateral blockade of MEK- and PI3K-mediated pathways downstream of mutant KRAS as a treatment approach for peritoneal mucinous malignancies.
Kuracha MR, Thomas P, Loggie BW, Govindarajan V.
PLoS One 2017 Jun; 12(6):e0179510.
Application：WB, Human, LS174T, RW7213 cells.
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miR-155 is positively regulated by CBX7 in mouse embryonic fibroblasts and colon carcinomas, and targets the KRAS oncogene.
Forzati F, De Martino M, Esposito F, Sepe R, Pellecchia S, Malapelle U, Pellino G, Arra C, Fusco A.
BMC Cancer 2017 Mar; 17(1):170.
Application：WB-Tr, Human, Mouse, A-549, HEK 293, MEF cells.
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Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10.
Madureira PA, Bharadwaj AG, Bydoun M, Garant K, O'Connell P, Lee P, Waisman DM.
Oncotarget 2016 Jul; 7(30):47720.
Application：WB-Tr, Human, A549, MiaPaca2 cells.
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Overexpression of DDX43 mediates MEK-Inhibitor Resistance through RAS up-regulation in Uveal Melanoma Cells.
Ambrosini G, Khanin R, Carvajal RD, Schwartz GK.
Molecular Cancer Therapeutics 2014 Aug; 13(8):2073.
Application：WB-Tr, Human, Omm1.3, Mel270 cells.
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Evidence for aldosterone-dependent growth of renal cell carcinoma.
King S, Bray S, Galbraith S, Christie L, Fleming S.
International Journal of Experimental Pathology 2014 Aug; 95(4):244.
Application：WB-Ce, WB-Ti, WB-Tr, Human, Renal cell carcinoma, RCC4 cells.
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KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy.
Valtorta E, Misale S, Sartore-Bianchi A, Nagtegaal ID, Paraf F, Lauricella C, Dimartino V, Hobor S, Jacobs B, Ercolani C, Lamba S, Scala E, Veronese S, Laurent-Puig P, Siena S, Tejpar S, Mottolese M, Punt CJ, Gambacorta M, Bardelli A, Di Nicolantonio F.
International Journal of Cancer 2013 Sep; 133(5):1259.
Application：FISH, Human, CRC cells.
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Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.
Fuentes-Calvo I, Blazquez-Medela AM, Santos E, Lopez-Novoa JM, Martinez-Salgado C.
PLoS One 2010 Jan; 5(1):e8703.
Application：WB, Human, Fibroblasts, Mesangial cells.
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Oncogenic KRAS mutation confers chemoresistance by upregulating SIRT1 in non-small cell lung cancer.
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