IL1B 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IL1B full-length
Host
Human
Theoretical MW (kDa)
30.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IL1B antibody (H00003553-B01) by Western Blots.
SDS-PAGE Gel
IL1B transfected lysate
Western Blot
Lane 1: IL1B transfected lysate ( 30.7 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IL1B
Entrez GeneID
3553GeneBank Accession#
NM_000576Protein Accession#
NP_000567Gene Name
IL1B
Gene Alias
IL-1, IL1-BETA, IL1F2
Gene Description
interleukin 1, beta
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq
Other Designations
catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
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