HCLS1 monoclonal antibody (M01), clone 3D5
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HCLS1.
Immunogen
HCLS1 (AAH16758, 266 a.a. ~ 355 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVE
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HCLS1 monoclonal antibody (M01), clone 3D5 Western Blot analysis of HCLS1 expression in K-562 ( Cat # L009V1 ).Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HCLS1 is approximately 3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HCLS1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — HCLS1
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Interactome
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Pathway
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Disease
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Publication Reference
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Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization.
Kan CY, Wen VW, Pasquier E, Jankowski K, Chang M, Richards LA, Kavallaris M, MacKenzie KL.
Oncogene 2012 Nov; 31(46):4815.
Application:WB-Ce, Human, Telomerase-immortalized bone marrow endothelial cells.
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Endothelial cell dysfunction and cytoskeletal changes associated with repression of p16(INK4a) during immortalization.
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