GDNF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GDNF transfected lysate.
Western Blot
Lane 1: GDNF transfected lysate ( 23.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GDNF full-length
Host
Human
Theoretical MW (kDa)
23.7
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GDNF antibody (H00002668-D01P) by Western Blots.
SDS-PAGE Gel
GDNF transfected lysate.
Western Blot
Lane 1: GDNF transfected lysate ( 23.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GDNF
Entrez GeneID
2668GeneBank Accession#
NM_000514Protein Accession#
NP_000505.1Gene Name
GDNF
Gene Alias
ATF1, ATF2, HFB1-GDNF
Gene Description
glial cell derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq
Other Designations
astrocyte-derived trophic factor|glial cell line derived neurotrophic factor|glial derived neurotrophic factor
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Interactome
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Disease
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