FCER1A monoclonal antibody (M01), clone 2C12-3B6
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More Files
- More Functions
Western Blot (Cell lysate)
FCER1A monoclonal antibody (M01), clone 2C12-3B6 Western Blot analysis of FCER1A expression in A-549 ( Cat # L025V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.
Lane 1: FCER1A transfected lysate(29.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (54.01 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FCER1A.
Immunogen
FCER1A (AAH05912.1, 1 a.a. ~ 257 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPLLVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRLLNPHPKPNPKNN
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.01 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FCER1A monoclonal antibody (M01), clone 2C12-3B6 Western Blot analysis of FCER1A expression in A-549 ( Cat # L025V1 ).Western Blot (Transfected lysate)
Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody (M01), clone 2C12-3B6.
Lane 1: FCER1A transfected lysate(29.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FCER1A is 1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi ( Cat # H00002205-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody (M01), clone 2C12-3B6 (Cat # H00002205-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — FCER1A
Entrez GeneID
2205GeneBank Accession#
BC005912Protein Accession#
AAH05912.1Gene Name
FCER1A
Gene Alias
FCE1A, FcERI
Gene Description
Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
Omim ID
147140Gene Ontology
HyperlinkGene Summary
The IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta (MIM 147138), and gamma (MIM 147139); only the alpha subunit is glycosylated.[supplied by OMIM
Other Designations
Fc IgE receptor, alpha polypeptide|Fc epsilon RI alpha-chain|Fc-epsilon RI-alpha|high affinity immunoglobulin epsilon receptor alpha-subunit|immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
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Interactome
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Pathway
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Disease
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