CDC25A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CDC25A transfected lysate.
Western Blot
Lane 1: CDC25A transfected lysate ( 59.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDC25A full-length
Host
Human
Theoretical MW (kDa)
59.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC25A antibody (H00000993-B01) by Western Blots.
SDS-PAGE Gel
CDC25A transfected lysate.
Western Blot
Lane 1: CDC25A transfected lysate ( 59.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDC25A
Entrez GeneID
993GeneBank Accession#
NM_001789Protein Accession#
NP_001780.2Gene Name
CDC25A
Gene Alias
CDC25A2
Gene Description
cell division cycle 25 homolog A (S. pombe)
Omim ID
116947Gene Ontology
HyperlinkGene Summary
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
M-phase inducer phosphatase 1|cell division cycle 25A|dual specificity phosphatase CDC25A
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Interactome
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Disease
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