CBS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CBS full-length
Host
Human
Theoretical MW (kDa)
60.6
Interspecies Antigen Sequence
Mouse (84); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CBS antibody (H00000875-B01) by Western Blots.
SDS-PAGE Gel
CBS transfected lysate.
Western Blot
Lane 1: CBS transfected lysate ( 60.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CBS
Entrez GeneID
875GeneBank Accession#
NM_000071.1Protein Accession#
-Gene Name
CBS
Gene Alias
HIP4
Gene Description
cystathionine-beta-synthase
Omim ID
236200Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq
Other Designations
OTTHUMP00000109416|OTTHUMP00000109418|beta-thionase|cystathionine beta-synthase|methylcysteine synthase|serine sulfhydrase
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Interactome
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Pathway
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Disease
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