CA12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CA12 transfected lysate.
Western Blot
Lane 1: CA12 transfected lysate ( 39.05 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CA12 full-length
Host
Human
Theoretical MW (kDa)
39.05
Interspecies Antigen Sequence
Mouse (82); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CA12 antibody (H00000771-B01) by Western Blots.
SDS-PAGE Gel
CA12 transfected lysate.
Western Blot
Lane 1: CA12 transfected lysate ( 39.05 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CA12
Entrez GeneID
771GeneBank Accession#
NM_001218.3Protein Accession#
NP_001209.1Gene Name
CA12
Gene Alias
CAXII, FLJ20151, HsT18816
Gene Description
carbonic anhydrase XII
Omim ID
603263Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
carbonic dehydratase
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Interactome
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Pathway
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