ATP6AP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ATP6AP1 transfected lysate.
Western Blot
Lane 1: ATP6AP1 transfected lysate ( 51.81 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP6AP1 full-length
Host
Human
Theoretical MW (kDa)
51.81
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP6AP1 antibody (H00000537-B01) by Western Blots.
SDS-PAGE Gel
ATP6AP1 transfected lysate.
Western Blot
Lane 1: ATP6AP1 transfected lysate ( 51.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP6AP1
Entrez GeneID
537GeneBank Accession#
NM_001183.4Protein Accession#
NP_001174.2Gene Name
ATP6AP1
Gene Alias
16A, ATP6IP1, ATP6S1, Ac45, CF2, MGC129781, VATPS1, XAP-3, XAP3
Gene Description
ATPase, H+ transporting, lysosomal accessory protein 1
Omim ID
300197Gene Ontology
HyperlinkGene Summary
This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1|ATPase, H+ transporting, lysosomal interacting protein 1|H-ATPase subunit|OTTHUMP00000032115|V-ATPase S1 accessory protein
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Interactome
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