ASNA1 monoclonal antibody (M03), clone 2H3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ASNA1.
Immunogen
ASNA1 (NP_004308, 239 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ASNA1 monoclonal antibody (M03), clone 2H3 Western Blot analysis of ASNA1 expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
ASNA1 monoclonal antibody (M03), clone 2H3. Western Blot analysis of ASNA1 expression in PC-12 ( Cat # L012V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody (M03), clone 2H3.
Lane 1: ASNA1 transfected lysate(38.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ASNA1 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi ( Cat # H00000439-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody (M03), clone 2H3 (Cat # H00000439-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ASNA1
Entrez GeneID
439GeneBank Accession#
NM_004317Protein Accession#
NP_004308Gene Name
ASNA1
Gene Alias
ARSA-I, ARSA1, MGC3821
Gene Description
arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Omim ID
601913Gene Ontology
HyperlinkGene Summary
ASNA1 is the human homolog of the bacterial arsA gene. In E. coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials.[supplied by OMIM
Other Designations
arsA arsenite transporter, ATP-binding, homolog 1
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Interactome
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Publication Reference
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Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions.
Ott M, Marques D, Funk C, Bailer SM.
Virology Journal 2016 Oct; 13(1):175.
Application:WB-Tr, Human, HeLa cells.
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A precursor specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum.
Rabu C, Wipf P, Brodsky JL, High S.
The Journal of Biological Chemistry 2008 Jul; 283(41):27504.
Application:WB, Human, HeLa cells.
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Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions.
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