AREG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AREG transfected lysate
Western Blot
Lane 1: AREG transfected lysate ( 27.83 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AREG full-length
Host
Human
Theoretical MW (kDa)
27.83
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AREG antibody (H00000374-B01) by Western Blots.
SDS-PAGE Gel
AREG transfected lysate
Western Blot
Lane 1: AREG transfected lysate ( 27.83 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AREG
Entrez GeneID
374GeneBank Accession#
BC009799Protein Accession#
AAH09799Gene Name
AREG
Gene Alias
AR, CRDGF, MGC13647, SDGF
Gene Description
amphiregulin
Omim ID
104640Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype. [provided by RefSeq
Other Designations
OTTHUMP00000160473|colorectum cell-derived growth factor|schwannoma-derived growth factor
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Interactome
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Pathway
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Disease
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