APRT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
APRT transfected lysate.
Western Blot
Lane 1: APRT transfected lysate ( 19.91 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-APRT full-length
Host
Human
Theoretical MW (kDa)
19.91
Interspecies Antigen Sequence
Mouse (83); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-APRT antibody (H00000353-B01) by Western Blots.
SDS-PAGE Gel
APRT transfected lysate.
Western Blot
Lane 1: APRT transfected lysate ( 19.91 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — APRT
Entrez GeneID
353GeneBank Accession#
NM_000485.2Protein Accession#
NP_000476.1Gene Name
APRT
Gene Alias
AMP, DKFZp686D13177, MGC125856, MGC125857, MGC129961
Gene Description
adenine phosphoribosyltransferase
Omim ID
102600Gene Ontology
HyperlinkGene Summary
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
AMP diphosphorylase|AMP pyrophosphorylase|adenine phosphoribosyltransferase, isoform a|transphosphoribosidase
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Interactome
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Pathway
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