ADA 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ADA full-length
Host
Human
Theoretical MW (kDa)
40.04
Interspecies Antigen Sequence
Mouse (83); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ADA antibody (H00000100-B01) by Western Blots.
SDS-PAGE Gel
ADA transfected lysate.
Western Blot
Lane 1: ADA transfected lysate ( 40.04 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ADA
Entrez GeneID
100GeneBank Accession#
NM_000022.2Protein Accession#
NP_000013.2Gene Name
ADA
Gene Alias
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Gene Description
adenosine deaminase
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq
Other Designations
adenosine aminohydrolase
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Interactome
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Pathway
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Disease
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